How To Determine Detection Limit (LoD) and Quantitation Limit (LoQ)

Thank you very much.

In case of AAS atomic absorption spectroscopy
The linearity start from 0.1 ppm to 0.5 ppm (LOD)
and the concentration fall below the 0.1 i.e 0.023 then
What will be the result?
Should we calculate this or not
If not then what will be the result?

If anyone have answer
Plz let me know

Hi sir
Why In visual observation LOQ not determined .
Ple send the answer

Hello Sir I got confused with how to compute the residuals especially the predictable response which you mentioned (mx + c). Kindly explain in detailed. And thank you in advance if you will notice my comment and query.

Why the number 3 comes in the formula for LOD?

Thank you👏🏼👏🏼👏🏼

Hello sir... according to ich guidelines loq value considered maximum 50% of our spec limit if our loq not stablish 50% or below spec limit than what will be do...??
Please suggest me sir...and explain what is the reason not stablish loq below 50%..

Great efforts

What if the specification limit is below to the quantification limit then is that LOQ is valid?

Is there need of seprate calibration curves for QL and DL.

It was very informative & useful information and you explained ina detail so anyone can understand, Great ..Help

Highly informative sir 👍🙏

Super sir

Tanks

hi Can you explain more about how to ca the calculate the residuals, I'm confused about the formula

Sir what is maximum limit of impurity

You made video on related substances

TQ Sir..

can we get the PPT PDF plz

Send me excel sheet sir plz.

Thank you so much sir

Great sir,your explanation is very versatile 👌, useful for us.

Very Informative lecture😍

Seperate curve for detection limit in that LOQ should not be added other linearity level can be added. Is it so

Actual value and predictable value explain with another one more example in visible manner

10.35 minutes can u explain a little elaborate with other example

Sir ur expalnation is very good but ihave a one doubt for blank mesurement method
1. We are inject blank solution or placebo 60 mesurements but the solutions not present in our analyte then how integrate peak and caliculate sddeviation

Your explanation is very powerful and easily understand by anyone. Appreciate.

Thank you sir, Highly informative. Please put video for gradient method development in HPLC

Why we determined lod and loq??

Sir please make a vidio in hindi language for better understanding.then will be more grow your channel and views and gain subscribers.

Sir please made a video for set the LOQ limit for 2 to 5 impurity present in sample having different uv
Please made all clear about the method validation with the example it will be helpful for us

ur explanation was amazing and crystal clear, keep making the lectures to enrich knowledge

Very helpful sir, can we have a video about impurity method validation from protocol preparation to report generation?

Which concentrations need to be selected while determine the lod and loq?can we go below 1 ppm too in the given example...

can i get it in pdf