How to calculate delta delta Ct in Excel

Your calculation is wrong. It should the the gene of interest - endogenous control, not the contrary. Refer to Livak, 2001.

Thank you!

Great video, very well explained

Can anyone help I have not duplicate values so I didn't take average

can we use reference gene as positive control

what is gene expression values and how to find or calculate them?

so what do the results mean? could you explain please? 43.9?